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human pancreatic epithelial cell line mia paca 2  (ATCC)


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    ATCC human pancreatic epithelial cell line mia paca 2
    Human Pancreatic Epithelial Cell Line Mia Paca 2, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epithelial+carcinoma+cell+line+atcc+crl/MIA+PaCa-2%3B+Pancreatic+Carcinoma%3B+Human/10__1007_slash_s43450___025___00666___4-61-17-45
    Average 95 stars, based on 81 article reviews
    human pancreatic epithelial cell line mia paca 2 - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: A Systems-Level Study Reveals Regulators of Membrane-less Organelles in Human Cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-PML (PG-M3) Santa Cruz Biotechnology sc-966; RRID: AB_628162 rabbit polyclonal anti-SRRM2 Sigma-Aldrich HPA041411; RRID: AB_10796671 mouse monoclonal anti-NPM1 Sigma-Aldrich B0556; RRID: AB_2154872 rabbit polyclonal anti-Coilin Santa Cruz Biotechnology sc-32860; RRID: AB_2081431 mouse monoclonal anti-G3BP1 Abcam ab56574; RRID: AB_941699 rabbit polyclonal anti-DDX6 Bethyl A300-461A; RRID: AB_2277216 rabbit monoclonal anti-RPA1/RPA70 Abcam ab79398; RRID: AB_1603759 rabbit polyclonal anti-Sp100 Sigma-Aldrich HPA017384; RRID: AB_1857399 mouse monoclonal anti-PABP Santa Cruz Biotechnology sc-32318; RRID: AB_628097 rabbit polyclonal anti-G3BP1 (H-94) Santa Cruz Biotechnology sc-98561; RRID: AB_2294329 mouse monoclonal anti-PML (1B9) Acris Antibodies AM26594AF-N donkey anti-mouse Alexa Fluor 488 ThermoFisher A-21202; RRID: AB_141607 donkey anti-rabbit Alexa Fluor 488 ThermoFisher A-21206; RRID: AB_141708 donkey anti-mouse Alexa Fluor 568 ThermoFisher A10037; RRID: AB_2534013 donkey anti-rabbit Alexa Fluor 568 ThermoFisher A10042; RRID: AB_2534017 donkey anti-mouse Alexa Fluor 647 ThermoFisher A-31571; RRID: AB_162542 Chemicals, Peptides, and Recombinant Proteins Hydroxyurea Sigma-Aldrich H8627 Thymidine Sigma-Aldrich T1895 Meayamycin Albert et al., 2009 N/A FR901464 Kaida et al., 2007 N/A alpha-Amanitin Sigma-Aldrich A2263 Critical Commercial Assays Click-iT EdU Alexa Fluor 647 Imaging Kit ThermoFisher # C10340 Deposited Data Raw images of all figures This paper; Mendeley Data https://doi.org/10.17632/h8byr7w3sx.1 Experimental Models: Cell Lines Human: HeLa, cervical cancer cell line Lab of Marino Zerial, Dresden, Germany HeLa MZ Human: A-431, epithelial carcinoma cell line ATCC CRL-1555 African green monkey: COS-7, kidney fibroblast-like cell line ATCC CRL-1651 Oligonucleotides siRNA targeting sequences: Plates 1-3 of the Ambion Silencer Select Human Druggable Genome siRNA Library V4 ThermoFisher #4397922 siRNA targeting sequences against selected human genes; Ambion Silencer Select This study N/A HIPK1 (NM_198268) cDNA GENEWIZ N/A HIPK2 (NM_022740) cDNA GENEWIZ N/A Recombinant DNA pEF1alpha-AcGFP1-C1 Vector Clontech #631974 pEF1alpha-AcGFP1-HIPK1 This paper N/A pEF1alpha-AcGFP1-HIPK2 This paper N/A (Continued on next page) e1 Molecular Cell 72, 1–15.e1–e5, December 20, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms MATLAB N/A https://www.mathworks.com/products/matlab.html Cytoscape N/A https://cytoscape.org/ GUI for pixel classification-based MLO segmentation This paper https://github.com/pelkmanslab/PixelClassification FlowSOM (algorithm for single cell clustering) Van Gassen et al., 2015 http://www.bioconductor.org/packages/release/bioc/ html/FlowSOM.html CellProfiler Carpenter et al., 2006 http://cellprofiler.org/ HIS (to calculate hierarchical interaction scores) Snijder et al., 2013 https://www.nature.com/articles/nmeth.2655# supplementary-information Cycler (to construct cell cycle trajectories) Gut et al., 2015 https://github.com/pelkmanslab/Cycler

    Imaging:

    Article Title: A Systems-Level Study Reveals Regulators of Membrane-less Organelles in Human Cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-PML (PG-M3) Santa Cruz Biotechnology sc-966; RRID: AB_628162 rabbit polyclonal anti-SRRM2 Sigma-Aldrich HPA041411; RRID: AB_10796671 mouse monoclonal anti-NPM1 Sigma-Aldrich B0556; RRID: AB_2154872 rabbit polyclonal anti-Coilin Santa Cruz Biotechnology sc-32860; RRID: AB_2081431 mouse monoclonal anti-G3BP1 Abcam ab56574; RRID: AB_941699 rabbit polyclonal anti-DDX6 Bethyl A300-461A; RRID: AB_2277216 rabbit monoclonal anti-RPA1/RPA70 Abcam ab79398; RRID: AB_1603759 rabbit polyclonal anti-Sp100 Sigma-Aldrich HPA017384; RRID: AB_1857399 mouse monoclonal anti-PABP Santa Cruz Biotechnology sc-32318; RRID: AB_628097 rabbit polyclonal anti-G3BP1 (H-94) Santa Cruz Biotechnology sc-98561; RRID: AB_2294329 mouse monoclonal anti-PML (1B9) Acris Antibodies AM26594AF-N donkey anti-mouse Alexa Fluor 488 ThermoFisher A-21202; RRID: AB_141607 donkey anti-rabbit Alexa Fluor 488 ThermoFisher A-21206; RRID: AB_141708 donkey anti-mouse Alexa Fluor 568 ThermoFisher A10037; RRID: AB_2534013 donkey anti-rabbit Alexa Fluor 568 ThermoFisher A10042; RRID: AB_2534017 donkey anti-mouse Alexa Fluor 647 ThermoFisher A-31571; RRID: AB_162542 Chemicals, Peptides, and Recombinant Proteins Hydroxyurea Sigma-Aldrich H8627 Thymidine Sigma-Aldrich T1895 Meayamycin Albert et al., 2009 N/A FR901464 Kaida et al., 2007 N/A alpha-Amanitin Sigma-Aldrich A2263 Critical Commercial Assays Click-iT EdU Alexa Fluor 647 Imaging Kit ThermoFisher # C10340 Deposited Data Raw images of all figures This paper; Mendeley Data https://doi.org/10.17632/h8byr7w3sx.1 Experimental Models: Cell Lines Human: HeLa, cervical cancer cell line Lab of Marino Zerial, Dresden, Germany HeLa MZ Human: A-431, epithelial carcinoma cell line ATCC CRL-1555 African green monkey: COS-7, kidney fibroblast-like cell line ATCC CRL-1651 Oligonucleotides siRNA targeting sequences: Plates 1-3 of the Ambion Silencer Select Human Druggable Genome siRNA Library V4 ThermoFisher #4397922 siRNA targeting sequences against selected human genes; Ambion Silencer Select This study N/A HIPK1 (NM_198268) cDNA GENEWIZ N/A HIPK2 (NM_022740) cDNA GENEWIZ N/A Recombinant DNA pEF1alpha-AcGFP1-C1 Vector Clontech #631974 pEF1alpha-AcGFP1-HIPK1 This paper N/A pEF1alpha-AcGFP1-HIPK2 This paper N/A (Continued on next page) e1 Molecular Cell 72, 1–15.e1–e5, December 20, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms MATLAB N/A https://www.mathworks.com/products/matlab.html Cytoscape N/A https://cytoscape.org/ GUI for pixel classification-based MLO segmentation This paper https://github.com/pelkmanslab/PixelClassification FlowSOM (algorithm for single cell clustering) Van Gassen et al., 2015 http://www.bioconductor.org/packages/release/bioc/ html/FlowSOM.html CellProfiler Carpenter et al., 2006 http://cellprofiler.org/ HIS (to calculate hierarchical interaction scores) Snijder et al., 2013 https://www.nature.com/articles/nmeth.2655# supplementary-information Cycler (to construct cell cycle trajectories) Gut et al., 2015 https://github.com/pelkmanslab/Cycler



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